Abstract:
The research work embodied in the present dissertation deals with isolation,
characterization and evaluation of biological activities of Indigofera heterantha, Millettia
ovalifolia, and selected conifers which are presented in five parts along with a brief
review of the biosynthesis of terpenoids, flavonoids, indigoferamide, indigoferate,
indigoferone, millettio peroxide and sesquiterpene.
PART- A
Part A describes the phytochemical and biological evaluation of Indigofera heterantha
(Wall). Three new and seven known compounds have been isolated from I. heterantha.
Various experimental techniques and extensive spectroscopic studies were used for the
isolation and structural elucidation of these compounds. Indigoferamide-A and
indigoferate showed significant activity, against selected bacteria, while indigoferone
exhibited high lipoxygenase activity.
New Chemical Constituents
Indigoferamide-A (12)
Indigoferate (13)
Indigoferone (14)
Known Chemical Constituents
Dotriacontanoic acid (15)
Formononetin (16)
Quercetin (17)
ii Quercetin 3-α-L-fucopyranoside (18)
3,5,7-Trihydroxy-6,4’-dimethoxyflavon (19)
3,5,4 ́-Trihydroxy-6,7-dimethoxyflavone (20)
Norartocarpetin (21)
4-Hydroxy-4-methyl-2-pentanone (22)
PART- B
Part B focused on isolation, purification, characterization and biological evaluation of the
chemical constituents of Millettia ovalifolia. Millettioperoxide along with five known
compounds have been isolated and characterized through physical characteristics and
extensive spectroscopic studies. The new compound possessed high anti-bacterial
activity.
New Chemical Constituents
Millettioperoxide (23)
Known Chemical Constituents
3,7-Dihydroxy-2-phenyl-4H-chromen-4-one (24)
(E)-Ethyl 13-(3,4-dimethoxyphenyl) acrylate (25)
(E)-Methyl 3-(3,4-dimethoxyphenyl) acrylate (26)
N-Ethylacetamide (27)
Benzoic acid (28)
iiiPART- C
Part C deals with the isolation and characterization of the chemical constituents of Abies
pindrow, which led to the isolation and structure elucidation of one new and four known
chemical constituents.
New Chemical Constituents
Abiespindrowpine (29)
Known Chemical Constituents
β-Sitosterol (30)
β-Sitosterol 3-O-β-D-glucopyranoside (31)
3β, 28-Dihydroxylup-20 (29)-ene (betulin) (32)
3β-Hydroxylup-20 (29)-ene-28-oic acid (betulinic acid) (33)
PART- D
Part D explains the isolation and characterization of phytochemical constituents of Pinus
wallichiana. Four known compounds have been isolated and characterized.
Known Chemical Constituents
β-Sitosterol (30)
β-Sitosterol 3-O-β-D-glucopyranoside (31)
5-Hydroxy-7-methoxy-2-(4-methoxy phenyl)-4H-chromen-4-one (34)
Oleanolic acid (35)
ivPART- E
Part E of the dissertation, described the isolation and structure elucidation of the chemical
constituents of Piceae smithiana. Two known compounds have been isolated and
characterized.
Known Chemical Constituents
β-Sitosterol (30)
β-Sitosterol 3-O-β-D-glucopyranoside (3